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Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis
Author(s) -
KHOKON MD. ATIQUR RAHMAN,
JAHAN MD. SARWAR,
RAHMAN TANIYA,
HOSSAIN MOHAMMAD ANOWAR,
MUROYAMA DAICHI,
MINAMI IKUKO,
MUNEMASA SHINTARO,
MORI IZUMI C.,
NAKAMURA YOSHIMASA,
MURATA YOSHIYUKI
Publication year - 2011
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.2011.02385.x
Subject(s) - abscisic acid , allyl isothiocyanate , methyl jasmonate , arabidopsis , myrosinase , glucosinolate , reactive oxygen species , chemistry , isothiocyanate , mutant , botany , biology , biochemistry , gene , brassica
Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects, pathogens and herbivores. In this study, we report that exogenously applied allyl isothiocyanate (AITC) induced stomatal closure in Arabidopsis via production of reactive oxygen species (ROS) and nitric oxide (NO), and elevation of cytosolic Ca 2+ . AITC‐induced stomatal closures were partially inhibited by an inhibitor of NADPH oxidase and completely inhibited by glutathione monoethyl ester (GSHmee). AITC‐induced stomatal closure and ROS production were examined in abscisic acid (ABA) deficient mutant aba2‐2 and methyl jasmonate (MeJA)‐deficient mutant aos to elucidate involvement of endogenous ABA and MeJA. Genetic evidences have demonstrated that AITC‐induced stomatal closure required MeJA priming but not ABA priming. These results raise the possibility that crucifer plants produce ITCs to induce stomatal closure, leading to suppression of water loss and invasion of fungi through stomata.