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Abscisic acid is involved in the response of grape ( Vitis vinifera L.) cv. Malbec leaf tissues to ultraviolet‐B radiation by enhancing ultraviolet‐absorbing compounds, antioxidant enzymes and membrane sterols
Author(s) -
BERLI FEDERICO J.,
MORENO DANIELA,
PICCOLI PATRICIA,
HESPANHOLVIANA LEANDRO,
SILVA M. FERNANDA,
BRESSANSMITH RICARDO,
CAVAGNARO J. BRUNO,
BOTTINI RUBÉN
Publication year - 2010
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.2009.02044.x
Subject(s) - vitis vinifera , abscisic acid , antioxidant , enzyme , ultraviolet b radiation , chemistry , ultraviolet , ultraviolet radiation , botany , biochemistry , biology , materials science , gene , optoelectronics , radiochemistry
We investigated the interactions of abscisic acid (ABA) in the responses of grape leaf tissues to contrasting ultraviolet (UV)‐B treatments. One‐year‐old field‐grown plants of Vitis vinifera L. were exposed to photosynthetically active radiation (PAR) where solar UV‐B was eliminated by using polyester filters, or where PAR was supplemented with UV‐B irradiation. Treatments combinations included weekly foliar sprays of ABA or a water control. The levels of UV‐B absorbing flavonols, quercetin and kaempferol were significantly decreased by filtering out UV‐B, while applied ABA increased their content. Concentration of two hydroxycinnamic acids, caffeic and ferulic acids, were also increased by ABA, but not affected by plus UV‐B (+UV‐B) treatments. Levels of carotenoids and activities of the antioxidant enzymes, catalase, ascorbate peroxidase and peroxidase were elevated by +ABA treatments, but only if +UV‐B was given. Cell membrane β ‐sitosterol was enhanced by ABA independently of +UV‐B. Changes in photoprotective compounds, antioxidant enzymatic activities and sterols were correlated with lessened membrane harm by UV‐B, as assessed by ion leakage. Oxidative damage expressed as malondialdehyde content was increased under +UV‐B treatments. Our results suggest that the defence system of grape leaf tissues against UV‐B is activated by UV‐B irradiation with ABA acting downstream in the signalling pathway.