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Metabolomic and physiological responses reveal multi‐phasic acclimation of Arabidopsis thaliana to chronic UV radiation
Author(s) -
LAKE JANICE A.,
FIELD KATIE J.,
DAVEY MATTHEW P.,
BEERLING DAVID J.,
LOMAX BARRY H.
Publication year - 2009
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.2009.02005.x
Subject(s) - acclimatization , arabidopsis thaliana , phenylpropanoid , trichome , botany , biology , arabidopsis , horticulture , biochemistry , biosynthesis , enzyme , mutant , gene
Biochemical changes in vivo and pathway interactions were investigated using integrated physiological and metabolic responses of Arabidopsis thaliana L. to ultraviolet (UV) radiation (280–400 nm) at 9.96 kJ m −2 d −1 over the entire life cycle from seed to seed (8 weeks). Columbia‐0 (Col‐0) and a UV‐B sensitive accession ( fah‐1 ) showed significant ( P < 0.001) reductions in leaf growth after 6 weeks. Col‐0 recovered growth after 8 weeks, with recovery corresponding to a switch from production of phenylpropanoids to flavonoids. fah‐1 failed to recover, indicating that sinapate production is an essential component of recovery. Epidermal features show that UV radiation caused significant ( P < 0.001) increases in trichome density, which may act as a structural defence response. Stomatal indices showed a significant ( P < 0.0001) reduction in Col‐0 and a significant ( P < 0.001) increase in fah‐1 . Epidermal cell density was significantly increased under UV radiation on the abaxial leaf surface, suggesting that that a fully functioning phenylpropanoid pathway is a requirement for cell expansion and leaf development. Despite wild‐type acclimation, the costs of adaptation lead to reduced plant fitness by decreasing flower numbers and total seed biomass. A multi‐phasic acclimation to UV radiation and the induction of specific metabolites link stress‐induced biochemical responses to enhanced acclimation.