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Extensive expression regulation and lack of heterologous enzymatic activity of the Class II trehalose metabolism proteins from Arabidopsis thaliana
Author(s) -
RAMON MATTHEW,
DE SMET IVE,
VANDESTEENE LIES,
NAUDTS MIRANDE,
LEYMAN BARBARA,
VAN DIJCK PATRICK,
ROLLAND FILIP,
BEECKMAN TOM,
THEVELEIN JOHAN M.
Publication year - 2009
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.2009.01985.x
Subject(s) - trehalose , biochemistry , arabidopsis thaliana , heterologous expression , biology , arabidopsis , yeast , heterologous , enzyme , function (biology) , phosphatase , gene , metabolism , mutant , microbiology and biotechnology , recombinant dna
Trehalose metabolism has profound effects on plant growth and metabolism, but the mechanisms involved are unclear. In Arabidopsis , 21 putative trehalose biosynthesis genes are classified in three subfamilies based on their similarity with yeast TPS1 (encoding a trehalose‐6‐phosphate synthase, TPS) or TPS2 (encoding a trehalose‐6‐phosphate phosphatase, TPP). Although TPS1 (Class I) and TPPA and TPPB (Class III) proteins have established TPS and TPP activity, respectively, the function of the Class II proteins (AtTPS5‐AtTPS11) remains elusive. A complete set of promoter‐ β ‐glucurinidase/green fluorescent protein reporters demonstrates their remarkably differential tissue‐specific expression and responsiveness to carbon availability and hormones. Heterologous expression in yeast furthermore suggests that none of the encoded enzymes displays significant TPS or TPP activity, consistent with a regulatory rather than metabolic function for this remarkable class of proteins.