Premium
Arabidopsis transcript and metabolite profiles: ecotype‐specific responses to open‐air elevated [CO 2 ]
Author(s) -
LI PINGHUA,
AINSWORTH ELIZABETH A.,
LEAKEY ANDREW D. B.,
ULANOV ALEXANDER,
LOZOVAYA VERA,
ORT DONALD R.,
BOHNERT HANS J.
Publication year - 2008
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.2008.01874.x
Subject(s) - ecotype , arabidopsis , metabolite , biology , arabidopsis thaliana , cape verde , microbiology and biotechnology , botany , gene , biochemistry , mutant , history , ethnology
A Free‐Air CO 2 Enrichment (FACE) experiment compared the physiological parameters, transcript and metabolite profiles of Arabidopsis thaliana Columbia‐0 (Col‐0) and Cape Verde Island (Cvi‐0) at ambient (∼0.375 mg g −1 ) and elevated (∼0.550 mg g −1 ) CO 2 ([CO 2 ]). Photoassimilate pool sizes were enhanced in high [CO 2 ] in an ecotype‐specific manner. Short‐term growth at elevated [CO 2 ] stimulated carbon gain irrespective of down‐regulation of plastid functions and altered expression of genes involved in nitrogen metabolism resembling patterns observed under N‐deficiency. The study confirmed well‐known characteristics, but the use of a time course, ecotypic genetic differences, metabolite analysis and the focus on clusters of functional categories provided new aspects about responses to elevated [CO 2 ]. Longer‐term Cvi‐0 responded by down‐regulating functions favouring carbon accumulation, and both ecotypes showed altered expression of genes for defence, redox control, transport, signalling, transcription and chromatin remodelling. Overall, carbon fixation with a smaller commitment of resources in elevated [CO 2 ] appeared beneficial, with the extra C only partially utilized possibly due to disturbance of the C : N ratio. To different degrees, both ecotypes perceived elevated [CO 2 ] as a metabolic perturbation that necessitated increased functions consuming or storing photoassimilate, with Cvi‐0 emerging as more capable of acclimating. Elevated [CO 2 ] in Arabidopsis favoured adjustments in reactive oxygen species (ROS) homeostasis and signalling that defined genotypic markers.