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Calcium pectate chemistry causes growth to be stored in Chara corallina : a test of the pectate cycle
Author(s) -
PROSEUS TIMOTHY E.,
BOYER JOHN S.
Publication year - 2008
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.2008.01829.x
Subject(s) - pectate lyase , calcium , chara , chemistry , botany , biochemistry , biology , enzyme , pectinase , organic chemistry
ABSTRACT Calcium pectate chemistry was reported to control the growth rate of cells of Chara corallina , and required turgor pressure ( P ) to do so. Accordingly, this chemistry should account for other aspects of growth, particularly the ability of plants to compensate for brief exposure to low P , that is, to ‘store’ growth. Live Chara cells or isolated walls were attached to a pressure probe, and P was varied. Low P caused growth to be inhibited in live cells, but when P returned to normal (0.5 MPa), a flush of growth completely compensated for that lost at low P for as long as 23–53 min. This growth storage was absent in isolated walls, mature cells and live cells exposed to cold, indicating that the cytoplasm delivered a metabolically derived growth factor needing P for its action. Because the cytoplasm delivered pectate needing P for its action, pectate was supplied to isolated walls at low P as though the cytoplasm had done so. Growth was stored while otherwise none occurred. It was concluded that a P ‐dependent cycle of calcium pectate chemistry not only controlled growth rate and new wall deposition, but also accounted for stored growth.