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CBF4 is a unique member of the CBF transcription factor family of Vitis vinifera and Vitis riparia
Author(s) -
XIAO HUOGEN,
TATTERSALL ELIZABETH A. R.,
SIDDIQUA MAHBUBA K.,
CRAMER GRANT R.,
NASSUTH ANNETTE
Publication year - 2008
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.2007.01741.x
Subject(s) - vitis vinifera , gene , biology , gene expression , transcription factor , botany , genetics
The CBF/DREB1 transcription factors control an important pathway for increased freezing and drought tolerance in plants. We report here the isolation of one CBF/DREB1 ‐like gene, CBF4 , from both freezing‐tolerant wild grape ( Vitis riparia ) and freezing‐sensitive cultivated grape ( Vitis vinifera ). The deduced protein in V. riparia is 99% identical to the corresponding protein in V. vinifera ; 45–48% to three other Vitis CBF proteins reported earlier and 57% to AtCBF1, and contains CBF‐specific amino acid motifs. Agroinfiltration experiments in tobacco leaves revealed that VrCBF4 activates expression from reporter genes driven by a CRT‐containing promoter. Expression of the endogenous Vitis CBF4 genes was low at ambient temperature, but enhanced upon exposure to low temperature (4 °C). Uncommon for CBF genes, this expression was maintained for several days. No significant difference in expression pattern was observed between V. riparia and V. vinifera . Vitis CBF4 was expressed in both young and mature tissue, in contrast to the previously described Vitis CBF1, 2 and 3 . Together, these results suggest that CBF4 represents a second type of CBF in grape that might be more important for the over‐wintering of grape plants.