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Quantitative trait loci controlling aluminium tolerance in two accessions of Arabidopsis thaliana (Landsberg erecta and Cape Verde Islands)
Author(s) -
KOBAYASHI YURIKO,
FURUTA YACHIYO,
OHNO TAKASHI,
HARA TETSUO,
KOYAMA HIROYUKI
Publication year - 2005
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.2005.01388.x
Subject(s) - quantitative trait locus , biology , cape verde , epistasis , genetics , population , arabidopsis thaliana , arabidopsis , inbred strain , allele , chromosome , transgressive segregation , chromosome 4 , gene , mutant , ethnology , demography , sociology , history
Quantitative trait loci (QTL) analysis of aluminium (Al) tolerance was performed using L er /Cvi recombinant inbred (RI) lines of Arabidopsis thaliana . Relative root length (RRL) (root length with 4 µ m Al/root length with no Al at pH 5.0) on day 5 was used as the Al tolerance index for QTL analysis. Al tolerance judged by RRL was well correlated to tolerance judged by other indexes, including accumulation of callose, reactive oxygen species in the root apex and growth performance on acid soil containing a large amount of exchangeable Al. Using data sets with an h b 2 of 0.91, two QTLs were detected at the top of chromosome 1 and bottom of chromosome 3. These QTLs explained 40 and 16% of the phenotypic variation of Al tolerance, respectively, and the positive effect of the Cvi allele. The QTL on chromosome 1 overlapped with a major QTL in another recombinant inbred population, and is possibly related to malate excretion. A complete pair‐wise search revealed 11 sets of epistatic interacting loci pairs, which accounted for the transgressive segregation among the RI population. Several epistatic interactions shared the same chromosomal region, indicating the possible involvement of regulatory proteins in Al tolerance in Arabidopsis .