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NO signalling in cytokinin‐induced programmed cell death
Author(s) -
CARIMI FRANCESCO,
ZOTTINI MICHELA,
COSTA ALEX,
CATTELAN IRENE,
DE MICHELE ROBERTO,
TERZI MARIO,
LO SCHIAVO FIORELLA
Publication year - 2005
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.2005.01355.x
Subject(s) - programmed cell death , nitric oxide , cytokinin , microbiology and biotechnology , arginine , biochemistry , reactive nitrogen species , biology , chemistry , apoptosis , reactive oxygen species , auxin , amino acid , gene , endocrinology
Cell death can be induced by cytokinin 6‐benzylaminopurine (BA) at high dosage in suspension‐cultured Arabidopsis cells. Herein, we provide evidence that BA induces nitric oxide (NO) synthesis in a dose‐dependent manner. A reduction in cell death can be observed when the cytokinin is supplemented with the NO scavenger 2‐(4‐carboxyphenyl)‐4,4,5,5‐tetramethylimidazoline‐1‐oxyl‐3‐oxide (cPTIO) or the nitric oxide synthase (NOS) inhibitors: 2‐aminoethyl‐isothiourea (AET) and N G. ‐monomethyl‐ l ‐arginine ( l ‐NMMA), which suggests that NO is produced via a NOS and is a signalling component of this form of programmed cell death. In BA‐treated cells, mitochondrial functionality is altered via inhibition of respiration. This inhibition can be prevented by addition of either cPTIO or AET implying that NO acts at the mitochondrial level.