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Multi‐element and multi‐site isotopic analysis of nicotine from tobacco leaves
Author(s) -
JAMIN E.,
NAULET N.,
MARTIN G. J.
Publication year - 1997
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1997.00099.x
Subject(s) - chemistry , deuterium , isotope , nicotine , isotope analysis , fractionation , isotopes of carbon , mass spectrometry , isotope fractionation , intramolecular force , kinetic isotope effect , nitrogen , stable isotope ratio , isotopes of nitrogen , isotope ratio mass spectrometry , δ13c , environmental chemistry , tobacco smoke , chromatography , organic chemistry , total organic carbon , geology , oceanography , physics , quantum mechanics , neuroscience , biology
The carbon, nitrogen and hydrogen stable‐isotope contents of nicotine extracted from tobacco leaves were determined by Isotope Ratio Mass Spectrometry (overall 2 H, 13 C and 15 N contents) and by the SNIF‐NMR method (site‐specific deuterium content). In addition, nicotine was chemically degraded into nicotinic acid so that the intramolecular distribution of carbon and nitrogen isotopes could be studied. A prerequisite for reliable measurements is the use of experimental procedures free of isotopic fractionation. Therefore, it was ensured that isotopic integrity was maintained throughout extraction, purification and chemical degradation steps. The multi‐element and multi‐site information provided is tentatively interpreted in terms of biochemical isotopic effects on intramolecular distribution patterns and of the influence of environmental factors on nicotine isotopic parameters.