Premium
Distribution and activity of microsomal NADPH‐dependent monooxygenases and amino acid decarboxylases in cruciferous and non‐cruciferous plants, and their relationship to foliar glucosinolate content
Author(s) -
BENNETT R. N.,
KIDDLE G.,
HICK A. J.,
DAWSON G. W.,
WALLSGROVE R. M.
Publication year - 1996
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1996.tb00417.x
Subject(s) - glucosinolate , monooxygenase , sinigrin , brassica , myrosinase , biochemistry , amino acid , raphanus , biology , botany , chemistry , enzyme , cytochrome p450
The NADPH‐dependent conversion of amino acids to their aldoximes is an initial step in glucosinolate biosynthesis. A number of microsomal aldoxime‐forming monooxygenase activities were detected in leaves from a variety of glucosi‐nolate‐containing species, whereas barley, bean and tobacco leaves did not contain any such activities. The substrates for these monooxygenases in each species largely correlated with the spectrum of glucosinolates found in that species. No activity was detected that metabolized homomethionine (supposed precursor of 2‐propenylglucosinolate [sinigrin]), even in species where sinigrin was the major glucosinolate. In Sinapis species containing hydroxybenzylglucosinolate (sinalbin), activity with L‐Tyr was detected, whereas Brassica species containing sinalbin had no such activity. However, these Brassicas did contain an L‐Phe monooxygenase activity. Partial characterization of the monooxygenases indicated that in Brassica species, Nasturtium officinalis and Raphanus sativus these resembled the flavin‐linked monooxygenases previously found in oilseed rape ( Brassica napus ) and Chinese cabbage ( Brassica campestris ). The L‐Tyr‐dependent activity in Sinapis species, and the L‐Phe‐dependent activity in Tropacolum majus , had characteristics of cytochrome P450‐type enzymes. No similarity was found with any other known amino acid metabolizing enzymes (including decarboxylases, amino acid oxidases and diamine/polyamine oxidases).