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Differential effects of Na + , Mg 2+ , K + , Ca 2+ and osmotic stress on the wild type and the NaCl‐tolerant mutants stl1 and stl2 of Ceratopteris richardii
Author(s) -
VOGELIEN D. L.,
HICKOK L. G.,
WARNE T. R.
Publication year - 1996
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1996.tb00222.x
Subject(s) - wild type , mannitol , osmotic shock , chemistry , sodium , mutant , biochemistry , gene , organic chemistry
In order to identify physiological components that contribute to salinity tolerance, we compared the effects of Na + , Mg 2+ and K + salts (NaCl, Na 2 SO 4 , MgCl 2 , MgSO 4 , KCl and K 2 SO 4 ), Ca 2+ (CaSO 4 ), mannitol and melibiose on the wild type and the single‐gene NaCl‐tolerant mutants stl1 and stl2 of Ceratopteris richardii . Compared with gametophytic growth of the wild type, stl2 showed a low level of tolerance that was restricted to Na + salts and osmotic stress. stl2 exhibited high tolerance to both Na + and Mg 2+ salts, as well as to osmotic stress. In response to short‐term exposure (3 d) to NaCl, accumulation of K + and Na + was similar in the wild type and stl1 . In contrast, stl2 accumulated higher levels of K + and lower levels of Na + . Ca 2+ supplementation (1.0 mol m −3 ) ameliorated growth inhibition by Na + and Mg 2+ stress in wild type and stll, but not in stl2 . In addition, under Na + stress (175 mol m −3 ) wild‐type, stll and stl2 gametopbytes maintained higher tissue levels of K + and lower levels of Na + when supplemented with Ca 2+ (1.0 mol m −3 ). stl2 gametophytes were extremely sensitive to K + supplementation. Growth of stl2 was greater than or equal to that of the wild type at trace concentrations of K + but decreased substantially with increasing K + concentration. Supplementation with K + from 0 to 1.85 mol m −3 alleviated some of the inhibition by 75 mol m −3 NaCl in the wild type and in stl1 . In stl2 , growth at 75 mol m −3 NaCl was similar at 0 and 1.85 mol m −3 K + supplementation. Although K + supplementation above 1.85 mol m −3 did not alleviate inhibition of growth by Na + in any genotype, stl2 maintained greater relative tolerance to NaCl at all K + concentrations tested.