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Evaluation of the potential to measure photosynthetic rates in C 3 plants ( Flaveria pringlei and Oryza sativa ) by combining chlorophyll fluorescence analysis and a stomatal conductance model
Author(s) -
HE D.,
EDWARDS G. E.
Publication year - 1996
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1996.tb00005.x
Subject(s) - stomatal conductance , photosynthesis , oryza sativa , chlorophyll fluorescence , botany , conductance , biology , fluorometer , fluorescence , physics , biochemistry , quantum mechanics , gene , condensed matter physics
The response curves of leaf photosynthesis to varying light, temperature and leaf‐to‐air vapour pressure deficit were measured in the C 3 plants Flaveria pringlei and Oryza sativa in normal air with a computerized open infrared gas analysis (IRGA) system, and the photochemical efficiency of photosystem II, described as (1– F ,/F′ m ) after Genty. Briantais & Baker (1989, Biochimica et Biophysica Acta 990, 87–92), was simultaneously measured with a modulated fluorometer. A model was written for rates of CO 2 fixation as a function of the true rate of O 2 evolution measured by fluorescene analysis ( J o 2 ), mesophyll conductance and intercellular CO 2 partial pressure. A second model was developed for rates of CO 2 fixation as a function of Jo 2 , mesophyll conductance and stomatal conductance. In the latter case, leaf stomatal conductance was simulated using the stomatal model proposed by Leuning (1995, Plant, Cell and Environment 18 , 339–355). The rates of CO 2 fixation predicted from the models were similar to rates measured by IRGA. The results indicate that there is potential to measure CO 2 fixation in C 3 plants by combining the non‐invasive measurement of Jo 2 by chlorophyll fluorescence analysis with the stomatal conductance model.