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Depolarization of tomato leaf cells by oligogalacturonide elicitors
Author(s) -
THAIN J.F.,
GUBB I.R.,
WILDON D. C.
Publication year - 1995
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1995.tb00355.x
Subject(s) - depolarization , cyanide , biophysics , membrane potential , chemistry , membrane , atpase , biology , botany , biochemistry , enzyme , inorganic chemistry
The electrical potential difference ( E m ) across the plasma membrane of tomato leaf mesophyll cells consists of a cyanide‐sensitive component, presumably produced by an H + ‐ATPase, and a cyanide‐insensitive component. Variation of E m between different batches of tissue is mainly caused by variation in the cyanide‐sensitive component. Oligogalacturonide elicitors that induce the synthesis of proteinase inhibitors in tomato seedlings depolarize the E m of tomato leaf mesophyll cells. This depolarization closely resembles that caused by cyanide: they are of similar magnitude and vary in a similar manner with variation in the initial E m of different batches of tissue. Treatments with cyanide and with the elicitors have similar effects on the small depolarization caused by KCl at 10 mol m −3 . The results suggest that the elicitors depolarize E m by inhibiting the plasma membrane H + ‐ATPase, but that the detailed mechanism of inhibition by the elicitors is different from that caused by cyanide.