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Effects of photoinhibitory treatment on CO 2 assimilation, the quantum yield of CO 2 assimilation, D 1 protein, ascorbate, glutathione and xanthophyll contents and the electron transport rate in vine leaves
Author(s) -
CHAUMONT M.,
MOROTGAUDRY J.F.,
FOYER C.H.
Publication year - 1995
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1995.tb00196.x
Subject(s) - photosynthesis , xanthophyll , photoinhibition , zeaxanthin , chemistry , quantum yield , botany , glutathione , horticulture , chloroplast , carotenoid , photosystem ii , biology , biochemistry , lutein , enzyme , physics , quantum mechanics , gene , fluorescence
The responses of photosynthesis to high light and low temperature were studied in vines cultivated in the greenhouse in low light. Exposure to high light (1000 /umol m −2 s −1 ) or low temperature (5 °C) alone had no measurable effect on the photosynthetic processes, but the combination of high light and low temperature caused rapid loss of photosynthetic capacity and a decrease in the efficiency of photosynthetic energy conversion. After a 15 h exposure to 5°C at high light, the F v /sb/F m ratio had decreased by 80% and the apparent quantum yield by 75%. Nevertheless, when the leaves were returned to low light at 22°C, these parameters recovered rapidly. The foliar pools of ascorbate and glutathione decreased in the first hours of photoinhibitory treatment while the zeaxanthin content increased from negligible levels to about 50% of the total foliar xanthophyll pool. There was a clear correlation between the zeaxanthin content of the leaves and their F v /F m ratio during both photoinhibition and recovery. However, there was also a good correlation between the decrease in theF v F m ratio and the measured decrease in the total foliar levels of the antioxidants ascorbate and glutathione. The amount of D, protein diminished over the same period as the zeaxanthin levels were increasing. This approach, involving simultaneous measurements of several parameters considered to influence photosystemy II activity, clearly demonstrates that measured decreases in F v /F m may not simply be related to zeaxanthin levels or to amounts of D 1 protein alone but result from multifactoral influences.