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Redox characteristics of normal and habituated cell lines of sugarbeet
Author(s) -
CARRIÉ B.,
GASPAR T.H.,
GREPPIN H.,
PENEL C.
Publication year - 1994
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1994.tb00315.x
Subject(s) - ferricyanide , redox , intracellular , dehydrogenase , oxidoreductase , enzyme , cell , biochemistry , in vitro , chemistry , biology , chemiluminescence , malate dehydrogenase , biophysics , chromatography , organic chemistry
Redox properties of cells of normal hormone‐requiring (N) and habituated (H) sugarbeet calli have been investigated. It was found that H cells at all ages reduced exogenous ferricyanide at a much higher rate than N cells, but they exhibited a lower chemiluminescence either alone or in presence of luminol. The plasma membrane NADH:fer‐ricyanide oxidoreductase measured in vitro was almost identical for the two cell types. This could indicate that the higher reducing power of H cells had an intracellular origin. Among the enzyme activities which could provide electrons, malate dehydrogenase activity was found to be a good candidate, being more active in H cells. The results are discussed in relation to the abnormal structure of the cell wall of habituated cells.