Premium
Growth cycle stage‐dependent NaCl induction of plasma membrane H + ‐ATPase mRNA accumulation in de‐adapted tobacco cells
Author(s) -
PEREZPRAT E.,
NARASIMHAN M. L.,
NIU X.,
BOTELLA M. A.,
BRESSAN R. A.,
VALPUESTA V.,
HASEGAWA P. M.,
BINZEL M. L.
Publication year - 1994
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1994.tb00299.x
Subject(s) - atpase , nicotiana tabacum , membrane , gene isoform , biochemistry , messenger rna , biology , chemistry , microbiology and biotechnology , enzyme , gene
A cDNA clone encoding an isoform of the plasma membrane H + ‐ATPase was isolated from Nicotiana tabacum. The steady‐state plasma membrane H + ‐ATPase message levels were the same in unadapted tobacco cells and tobacco cells adapted to 428 mol m −3 NaCl. When cells adapted to 428 mol m −3 NaCl maintained in the absence of NaCl (deadapted) for an excess of 100 passages were exposed to 400 mol m −3 NaCl for 24 h, there was an increased accumulation of plasma membrane H + ‐ATPase message. The NaCl responsiveness of the deadapted cells was dependent upon the growth cycle stage. Alterations in the levels of plasma membrane FT‐ATPase message during the growth cycle support a role for the H + ‐ATPase in cell growth. These results document the induction by NaCl of plasma membrane FT‐ATPase message accumulation in tobacco cells, and suggest that enhanced expression of the plasma membrane FT‐ATPase has a role in the short term response of cells of NaCl, but is not necessarily involved in long‐term adaptation.