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Effect of Ca 2+ on the retention of glycerol and amino acids in Dunaliella tertiolecta
Author(s) -
FUJII S.
Publication year - 1994
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1994.tb00287.x
Subject(s) - glycerol , putrescine , intracellular , spermine , amino acid , egta , chemistry , spermidine , chromatography , biochemistry , nuclear chemistry , calcium , organic chemistry , enzyme
When D. tertiolecta cells, previously incubated in a 0.5 kmol m −3 NaCl medium with 1mol m −3 Ca 2+ , were transferred to an isotonic NaCl medium without Ca 2+ , the intracellular glycerol, as well as intracellular amino acids, was transiently lost to the medium within 30 min. The transient leakage of glycerol and amino acids was enhanced by the addition of EGTA (1 mol m −3 ), while the addition of SrCl 2 (1 mol m −3 ) or polyamines such as spermidine (5 mol m −3 ) and spermine (5 mol m −3 ) restrained the leakage caused by the lack of external Ca 2+ of intracellular glycerol and amino acids.