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Metabolic control analysis of plant metabolism
Author(s) -
Ap REES T.,
HILL S. A.
Publication year - 1994
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1994.tb00151.x
Subject(s) - flux (metallurgy) , metabolism , substrate (aquarium) , plant metabolism , metabolic control analysis , control (management) , chemistry , metabolic flux analysis , biological system , computer science , biochemical engineering , environmental science , control theory (sociology) , biochemistry , engineering , biology , microbiology and biotechnology , ecology , rna , organic chemistry , gene , insulin , artificial intelligence
Metabolic control analysis and its major coefficients are introduced. The importance of measuring both elasticity and concentration‐control coefficients as well as flux‐control coefficients is stressed. The conditions that need to be met before control analysis can be applied experimentally are emphasized. It is argued that successful application of this approach requires methods for the measurement of flux, maximum catalytic activities of enzymes and substrate contents. The measurement of flux by consumption of substrate, production of product, and the distribution of isotope after metabolism of labelled substrates is discussed. The need to ensure that measurements of enzymes and substrates are reliable and authenticated is stressed. Particular emphasis is placed on the ease with which such measurements can be invalidated in plant tissues, and ways for countering these difficulties are discussed.