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The effects of enhanced ozone and enhanced carbon dioxide concentrations on biomass, pigments and antioxidative enzymes in spruce needles ( Picea abies L.)
Author(s) -
POLLE A.,
PFIRRMANN T.,
CHAKRABARTI S.,
RENNENBERG H.
Publication year - 1993
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1993.tb00874.x
Subject(s) - picea abies , ozone , catalase , superoxide dismutase , chemistry , shoot , horticulture , peroxidase , botany , oxidative stress , enzyme , biology , biochemistry , organic chemistry
During one growing period, 5‐year‐old spruce trees (Picea abies L., Karst.) were exposed in environmental chambers to elevated concentrations of carbon dioxide (750 cm 3 m −3 ) and ozone (008 cm 3 m −3 ) as single variables or in combination. Control concentrations of the gases were 350cm 3 m −3 CO 2 and 0.02 cm 3 m −3 ozone. To investigate whether an elevated CO 2 concentration can prevent adverse ozone effects by reducing oxidative stress, the activities of the protective enzymes superoxide dismutase, catalase and peroxidase were determined. Furthermore, shoot biomass, pigment and protein contents of two needle age classes were investigated. Ozone caused pigment reduction and visible injury in the previous year's needles and growth reduction in the current year's shoots. In the presence of elevated concentrations of ozone and CO 2 , growth reduction in the current year's shoots was prevented, but emergence of visible damage in the previous year's needles was only delayed and pigment reduction was still found. Elevated concentrations of ozone or CO 2 as single variables caused a significant reduction in the activities of superoxide dismutase and catalase in the current year's needles. Minimum activities of superoxide dismutase and catalase and decreased peroxidase activities were found in both needle age classes from spruce trees grown at enhanced concentrations of both CO 2 and ozone. These results suggest a reduced tolerance to oxidative stress in spruce trees under conditions of elevated concentrations of both CO 2 and ozone.