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Photoinhibition of isolated mesophyll cells from cold‐hardened and non‐hardened winter rye
Author(s) -
LAPOINTE L.,
HUNER N. P. A.
Publication year - 1993
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1993.tb00867.x
Subject(s) - photoinhibition , secale , botany , biology , horticulture , cold stress , chlorophyll , photosynthesis , photosystem ii , biochemistry , gene
Cold‐hardened rye leaves have been shown to be more resistant to low temperature photoinhibition than non‐hardened rye leaves. Isolated mesophyll cells from winter rye (Secale cereale L. cv. Musketeer) were exposed to photoinhibitory light conditions to estimate the importance of leaf morphology and leaf optical properties in the resistance of cold‐hardened rye leaves to photoinhibition. Cold‐hardened rye cells showed more resistance to photoinhibition than non‐hardened rye cells when monitored with chlorophyll a variable to maximal fluorescence ratio (F v /F m ). Thus, leaf morphology does not contribute to the resistance of cold‐hardened rye leaves to low temperature photoinhibition. However, cold‐hardened and non‐hardened rye cells showed a similar extent of photoinhibition when photsynthetic CO 2 fixation rates were measured. They also showed the same capacity to recover from photoinhibition. During both photoinhibition and recovery, F v /F m and light limited CO 2 fixation rates showed different kinetics. We propose that inactivation and subsequent reactivation during recovery of some light activated Calvin cycle enzymes explain the greater extent of photoinhibition of light limited CO 2 fixation and its faster recovery compared to F v /F m kinetics during photoinhibition.