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Changes in ethylene and polyamines in relation to mRNA levels of the large and small subunits of ribulose bisphosphate carboxylase/oxygenase in ozone‐stressed potato foliage
Author(s) -
REDDY G. NAGI,
ARTECA R. N.,
DAI Y.R.,
FLORES H. E.,
NEGM F. B.,
PELL E. J.
Publication year - 1993
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1993.tb00503.x
Subject(s) - ribulose 1,5 bisphosphate , putrescine , rubisco , spermine , spermidine , oxygenase , polyamine , ethylene , pyruvate carboxylase , abscisic acid , chemistry , biochemistry , biology , botany , enzyme , gene , catalysis
Potato plants ( Solanum tuberosum L. cv‘Norland’) were subjected to acute and chronic exposures of ozone (O 3 ). Periodically following or during the exposures, foliage of different physiological ages were sampled for tissue analysis. Measurement of the following parameters were included: ethylene, 1‐aminocyclopropane‐1‐carboxylic acid (ACC), N‐malonyl 1‐aminocyclopropane‐1‐carboxylic acid (MACC), and ACC oxidase activity, putrescine, spermidine, and spermine levels, and ornithine decarboxylase (ODC) activity, and levels of mRNA transcripts for the large and small subunits of ribulose 1,5‐bisphosphate carboxylase/oxygenase (Rubisco) ( rbc L and rbc S, respectively). Ozone increased emission of ethylene and the concentration and activity of all associated metabolites and enzymes. Putrescine titre increased in response to O 3 as did ODC activity. Some increases in spermidine were also detected. Ozone decreased the levels of mRNA for rbc L and rbc S, with the latter transcript exhibiting greater sensitivity. After acute exposures were terminated, the rbc L and rbc S transcript in younger leaves returned to levels of nonstressed plants; effects were less likely to be reversed in older tissue. The potential relationship between the changes in rbc L and rbc S and ethylene and polyamines are discussed in the context of O 3 induction of accelerated senescence.