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Sugarcane proteins and messenger RNAs regulated by salt in suspension cells
Author(s) -
RAMAGOPAL S.,
CARR J. B.
Publication year - 1991
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1991.tb01370.x
Subject(s) - gene expression , messenger rna , salinity , cell culture , microbiology and biotechnology , gene , biology , cell , biochemistry , genetics , ecology
Abstract. Changes in gene expression induced by salinity were investigated in a suspension culture of the tropical, graminaceous plant sugarcane ( Sacchamm spp.). Three cell lines tolerant to 5, 10 and 15kg m −3 NaCl were adapted and the expression of newly synthesized proteins and translatable mRNAs in these cell lines were compared with those in unadapted cells on high‐resolution, two‐dimensional gels. Specific proteins and mRNAs altered by salinity were identified. At the protein level, the expression of 15 proteins was induced or enhanced, and that of three others was repressed or abolished. At the translatable mRNA level, the expression of 18 mRNAs was induced or enhanced, and that of eight others was repressed or abolished. The expression of some of these was also regulated, depending on the salt‐adapted cell line. The above changes were characteristic of salt‐adapted cells and were not found in unadapted cells undergoing a short‐term salt stress. Overall, 29 sugarcane proteins were regulated by salinity: for 15, translatable mRNAs were found; for three, no translatable mRNA was detected; and for six of the remaining 11, in vivo equivalents were not observed. These data suggest that a multitude of mechanisms at the transcriptional, post‐transcriptional and post‐translational levels may contribute to the control of gene expression in the sail‐adapted sugarcane cells.