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The perturbation of the endomembrane network of the chlamydomonad alga Gloeomonas by the herbicide isopropyl N‐phenyl carbamate (IPC)
Author(s) -
DOMOZYCH D. S.
Publication year - 1991
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1991.tb00948.x
Subject(s) - endomembrane system , chemistry , microbiology and biotechnology , nocodazole , isopropyl , biophysics , biochemistry , cell , biology , golgi apparatus , medicinal chemistry , cytoskeleton
. The highly complex and symmetric endomembrane network of the chlamydomonad alga, Gloeomonas kupferri , can be disrupted by the herbicide, isopropyl N‐phenyl carbamate (IPC). Upon incubation with IPC for between 30 min and 1 h, curling of the dictyosomes, proliferation of small vesicles from the trans face and swelling of the peripheral vacuolar complex takes place. Longer treatments result in degeneration of dictyosome cisternae into vesicular masses and the formation of supervacuolar complexes. Enzyme cytochemistry revealed that endomembrane ‘marker’ enzymes are altered/inhibited by IPC. A second but striking I PC‐induced effect is the production of branched, fibrillar inclusions in the cell nucleus. A discussion of I PC‐induced affects on ion regulation and microtubule/MTOC distribution in the cell is presented.