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The active uptake of carbon dioxide by the unicellular green algae Chlorella saccharophila and C. ellipsoidea
Author(s) -
ROTATORE C.,
COLMAN B.
Publication year - 1991
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1991.tb00945.x
Subject(s) - carbonic anhydrase , chlorella , carbon dioxide , photosynthesis , chemistry , algae , green algae , acetazolamide , nuclear chemistry , pigment , biochemistry , alkali metal , food science , botany , enzyme , biology , organic chemistry , physiology
. Mass spectrometry has been used to measure the rates of CO 2 uptake of acid‐ and alkali‐grown cells of the green algae Chlorella ellipsoidea (UTEX 20) and C. saccharophila (UTEX 27). The time course of CO 2 formation on addition of 100mmol m −3 K 2 CO 3 to cells in the dark was used as an assay for external carbonic anhydrase (CA). No external CA was detected in acid‐grown cells of either species or in alkali‐grown cells of C. ellipsoidea but was present in alkali‐grown C. saccharophila . In the absence of external CA, or when it was inhibited by 5mmol m −3 acetazolamide, cells of both species, on illumination, rapidly depleted the free CO 2 in the medium at pH 7.5 to near zero concentrations before maximum photosynthetic O 2 evolution rates were established. Addition of bovine CA rapidly restored the equilibrium CO 2 concentration in the medium, indicating that the cells were selectively taking up CO 2 . Transfer of cells to the dark caused a rapid increase in the CO 2 concentration in the medium largely due to the efflux of inorganic carbon from the cells as CO 2 . This rapid light‐dependent CO 2 uptake takes place against pH and concentration gradients and, thus, has the characteristics of active transport.