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Survival and death of Chara internodal cells in electrolyte solutions and calcium release from the cell wall
Author(s) -
KIYOSAWA K.,
ADACHI T.
Publication year - 1990
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1990.tb01324.x
Subject(s) - chemistry , calcium , potassium , hepes , alkali metal , tris , salt (chemistry) , phosphate , buffer solution , inorganic chemistry , electrolyte , potassium phosphate , nuclear chemistry , sodium , chromatography , biochemistry , organic chemistry , electrode
Abstract. Survival and death of Chara internodal cells were investigated in one of the alkali metal salts KCl, some of the alkali earth metal salts CaCl 2 , Ca(NO 3 ) 2 , MgCl 2 , Mg(NO 3 ) 2 , SrCl 2 , Sr(NO 3 ) 2 , BaCl 2 and Ba(NO 3 ) 2 , potassium phosphate pH buffer solution (pH 7.0), Tris‐maleate pH buffer solution (pH 7.0), HEPES (N‐2‐hydroxyethylpiperazine‐N′‐2‐ethanesulphonic acid)‐KOH (pH 7.0) pH buffer solution, calcium buffer solutions, and deionized water. Most of the internodal cells died within a day or a few days in KCl, MgCl 2 , Mg(NO 3 ) 2 , BaCl 2 and Ba(NO 3 ) 2 solutions of higher concentrations, calcium buffer solutions of pCa 6.0, 10.0 mol m ‐3 potassium phosphate pH buffer solution and 10.0 mol m ‐3 Trismaleate pH buffer solution. However, all of the internodal cells survived more than 10 d in deionized water, 80.0 mol m ‐3 CaCl 2 , 80.0 mol m ‐3 Ca(NO 3 ) 2 , 80.0 mol m ‐3 SrCl 2 , 80.0 mol m ‐3 Sr(NO 3 ) 2 calcium buffer solutions of pCa 4.0 and pCa 5.0, and 10.0 mol m ‐3 HEPES‐KOH (pH 7.0) pH buffer solution. Addition of Ca 2+ or Sr 2+ to K + , Mg 2+ and Ba 2+ salt solutions increased the survival rates of the internodal cells. Calcium release from the internodal cell wall was measured in deionized water, KCl, NaCl, MgCl 2 , CaCl 2 , SrCl 2 and BaCl 2 solutions. Except in deionized water and CaCl 2 solution, most of the calcium binding to the cell wall was released within one or a few hours in respective electrolyte solutions. Thus, survival and death of the internodal cells in the electrolyte solutions tested were interpreted in terms of the calcium release from the cell wall and the cell membrane, and intrinsic ability of Sr 2+ to maintain the cell membrane normal.