Premium
Changes in glutamate dehydrogenase activity of Chlamydomonas reinhardtii under different trophic and stress conditions
Author(s) -
MUÑOZBLANCO J,
CARDENAS J.
Publication year - 1989
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1989.tb01930.x
Subject(s) - glutamine synthetase , glutamate dehydrogenase , glutamate synthase , chlamydomonas reinhardtii , biochemistry , ammonium , glutamate receptor , glutamine , biology , chlamydomonas , metabolism , chemistry , amino acid , receptor , organic chemistry , gene , mutant
. Under stress conditions (darkness, nitrogen starvation, high ammonium concentrations, glutamine synthetase and glutamate synthase inhibition) glutamate dehydrogenase animating activity levels of Chlamydomonas cells varied inversely to those of glutamine synthetase. Nitrogen and carbon sources also influenced glutamate dehydrogenase levels in Chlamydomonas , the highest values being found in cells cultured mixotrophically with ammonium, under which conditions glutamate dehydrogenase and glutamine synthetase levels were likewise inversely related. These facts, together with the analysis of internal fluctuations of ammonium, 2‐oxoglutarate, and the amino acid pool as well as the variations of certain enzymes involved in carbon metabolism indicate that glutamate dehydrogenase animating activity is adaptative, being involved in the maintenance of intracellular levels of L‐glutamate when they cannot be maintained by the GS‐GOGAT cycle, and probably more connected with carbon than nitrogen metabolism.