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Site‐directed monoclonal antibodies against the amino terminus of 124‐kDa phytochrome from Avena sativa L.
Author(s) -
PLUMPTON C.,
PARTIS M. D.,
THOMAS B.,
HUSKISSON N. S.,
BUTCHER G. W.
Publication year - 1988
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1988.tb01787.x
Subject(s) - phytochrome , monoclonal antibody , antibody , peptide , microbiology and biotechnology , avena , biochemistry , biology , epitope , chemistry , botany , immunology , red light
Two monoclonal antibodies (AFRC MAC 184 and 185) have been raised in rats against a synthetic octadecapeptide corresponding to the N‐terminus of Avena phytochrome. The peptide was conjugated to tuberculin purified protein derivative (PPD) for immunization and the cell lines screened by ELISA using the free peptide. Both antibodies bind to intact 124‐kDa phytochrome on Western blots and in a double antibody sandwich ELISA. In the ELISA, they have an approximately four‐fold higher affinity for Pr than Pfr. Conformational changes during photoconversion, therefore, involve the extreme N‐terminus of the phytochrome molecule.