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Translatable mRNA changes in ethylene induced abscission zones of Phaseolus vulgaris (Red Kidney)
Author(s) -
KELLY P.,
TREWAVAS A. J.,
LEWIS L. N.,
DURBIN M. L.,
SEXTON R.
Publication year - 1987
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1987.tb02074.x
Subject(s) - phaseolus , abscission , petiole (insect anatomy) , messenger rna , biology , ethylene , rna , biochemistry , botany , microbiology and biotechnology , chemistry , gene , hymenoptera , catalysis
The wheat germ translation system was programmed with soluble RNA extracted from foliar abscission zones of Phaseolus vulgaris , These extracts were taken at various times after the induction of abscission. A translation product with a molecular weight of 42 kilodalton (kD) was only present after this treatment, though three other species 32, 27 and 17 kD increased substantially. The isozyme of cellulase with a pi of 9.5 could not be conclusively identified amongst the products though the 32 kD protein is probably chitinase. Comparison of the abscission zone translatable RNA with that from adjacent petiole and stem tissues showed the 17 kD protein developed in all these location. The 42, 32 and 27 kD bands were found predominantly in the zone and petiole.