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CAM induction by photoperiodism in green callus cultures from a CAM plant
Author(s) -
BRULFERT J.,
MRICHA A.,
SOSSOUNTZOV L.,
QUEIROZ O.
Publication year - 1987
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1987.tb01822.x
Subject(s) - callus , phytotron , photoperiodism , kalanchoe , biology , botany , horticulture , crassulaceae , panicle , crassulacean acid metabolism , photosynthesis
Green calli obtained from leaves of the CAM‐inducible plant Kalanchoe blossfeldiana cv. Montezuma were grown either under long‐day or short‐day regimes in the Phytotron of Gif‐sur‐Yvette. Callus cells were found to be CAM inducible by the short‐day treatment, according to levels and diurnal oscillations of malate pools and phosphor‐enolpyruvate (PEP) carboxylase (EC.4.1.1.31) capacity. De novo synthesis of PEP carboxylase was shown to occur under the short‐day regime. In spite of continuous net CO 2 output, CAM‐like patterns of CO 2 exchange by calli were obtained under the short‐day treatment. After 2 months under both photoperiodic conditions, spontaneous organogenesis in callus tissues gave rise to numerous shootlets which were determined as photoperiod dependent for CAM; the same was so for plants originating from these shootlets.