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Survival of Cornus sericea L. stem cortical cells following immersion in liquid helium
Author(s) -
GUY C. L.,
NIEMI K. J.,
FENNELL A.,
CARTER J. V.
Publication year - 1986
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1986.tb01759.x
Subject(s) - horticulture , parenchyma , biology , botany , zoology , chemistry
Cold‐acclimated stems of red‐osier dogwood ( Cornus sericea L.) were sampled in midwinter and early spring and subjected to the following low temperature treatments: (a)0 →−40 → 0°C; (b) 0 →−40 →− 196 → 0°C; (c) 0 →−40 →−196 →−269 →−196 → 0°C; (d) 0 →−40 →−269 →−196 → 0°C; (e) 0 →−196 → 0°C; (f) 0 →−269 →−196 →0°C. The cortical parenchyma cells of the outer stem layers survived exposure to −269°C when pre‐frozen to −40°C and either transferred directly to −269°C or to −196°C and then to −269°C (treatments c and d). Acclimated stems transferred to a greenhouse (22°C) 2 weeks prior to the low temperature treatments deacclimated and were not able to survive freezing to −10°C. Cortical cells of stem samples taken in March, near the time when dogwood naturally deacclimates, survived −196°C (treatment b), but not −269°C (treatment cord). Thus, the freezing tolerance of dogwood varies seasonally from near −10°C to below −269°C.