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A procedure for isolating vacuoles from leaves of the halophyte Suaeda maritima
Author(s) -
DRACUP M. N. H.,
GREENWAY H.
Publication year - 1985
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1985.tb01222.x
Subject(s) - vacuole , protoplast , halophyte , biology , staining , biochemistry , mesembryanthemum crystallinum , chenopodiaceae , reductase , enzyme , cytoplasm , ecology , genetics , salinity
. A method is described here for isolating protoplasts and vacuoles from leaves of the halophyte Suacda maritima. Integrity of the protoplasts and vacuoles was tested by staining and shown to be more than 75%, while use of biochemical markers, staining and light microscopy suggested a high degree of purity of the vacuoles. Phosphatase and NADH cytochrome‐ c ‐reductase were associated with vacuoles; phosphatase showed an eight‐fold enrichment and NADH cytochrome‐ c ‐reductase a 3.5‐fold enrichment relative to protoplasts. The vacuoles contained only 15% of the protein in protoplasts.