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An apparatus for pulse and pulse‐chase experiments with 14 CO 2 on attached leaves with known, steady‐state rates of photosynthesis
Author(s) -
JONES D. ALAN,
SMITH ALISON M.,
WOOLHOUSE HAROLD W.
Publication year - 1983
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1983.tb01889.x
Subject(s) - photosynthesis , pulse (music) , steady state (chemistry) , flux (metallurgy) , chemistry , photon flux , analytical chemistry (journal) , botany , atomic physics , physics , photon , optics , biology , chromatography , organic chemistry , detector
. An apparatus is described to carry out pulse and pulse‐chase experiments with 14 CO 2 on intact, attached leaves with known, steady‐state rates of photosynthesis under defined conditions of temperature, vapour pressure deficit and photon flux density. Data are presented which show that the pattern of distribution of 14 C between compounds in extracts of such leaves is a true reflection of the pathways of photosynthetic carbon metabolism in the leaf during steady‐state photosynthesis.