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Effects of light on the catabolism of [2‐ 14 C]‐3‐indole acetic acid in protoplasts, a chloroplast‐rich fraction, and a crude cytoplasmic preparation from barley ( Hordeum vulgare L)
Author(s) -
SANDBERG GÖRAN,
JENSEN EINAR,
CROZIER ALAN
Publication year - 1983
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1983.tb01883.x
Subject(s) - catabolism , protoplast , chloroplast , indole 3 acetic acid , hordeum vulgare , acetic acid , biochemistry , biology , fraction (chemistry) , darkness , chromatography , chemistry , metabolism , botany , poaceae , auxin , gene
. Combined gas chromatography‐mass spectrometry has been used to identify 3‐indole acetic acid in an extract from protoplasts of barley. In addition, a study has been made of the effect of light on the rate of catabolism of [2‐ 14 C]‐3‐indole acetic acid by protoplasts, a chloroplast‐rich fraction and a crude cytoplasmic preparation from barley leaves. While light enhanced the rate of catabolism of [2‐ 14 C]‐3‐indok acetic acid by protoplasts and, to a lesser degree, by the chloroplast‐rich fraction, it did not affect the catabolic activity of the crude, cytoplasmic fraction. These findings, when considered along with the data of Sandberg, Jensen & Crozier (1983), imply that the rate of turnover of 3‐indole acetic acid, in both protoplasts and chloroplasts, is more rapid in light than it is in darkness.