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Transport of purines and pyrimidines into isolated pea chloroplasts
Author(s) -
BARBER D. J.,
THURMAN D. A.
Publication year - 1978
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/j.1365-3040.1978.tb02044.x
Subject(s) - chloroplast , purine metabolism , cytosine , chemistry , biophysics , biochemistry , biology , dna , gene , enzyme
. Uptake of [ 14 C] adenine and [ 14 C] cytosine into isolated pea chloroplasts has been examined by using a centrifugal‐filtration technique. These chloroplasts accumulate both compounds in the sucrose‐impermeable space to concentrations higher than that present in the external solution when the latter was below 1.0 mM. Competition experiments suggested that uptake is mediated by a carrier mechanism.