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Quantitative method for pheromone delivery in studies of sensory adaptation of moth antennae
Author(s) -
TRIMBLE R. M.,
MARSHALL D. B.
Publication year - 2007
Publication title -
physiological entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 57
eISSN - 1365-3032
pISSN - 0307-6962
DOI - 10.1111/j.1365-3032.2007.00591.x
Subject(s) - pheromone , ethanol , biology , airflow , zoology , botany , food science , biochemistry , mechanical engineering , engineering
A pheromone sprayer and an electroantennogram (EAG) are used to study sensory adaptation in the antennae of male obliquebanded leafrollers, Choristoneura rosaceana and oriental fruit moths, Grapholita molesta , to the main pheromone compounds ( Z )‐11‐tetradecen‐1‐yl acetate ( Z 11‐14:Ac) and ( Z )‐8‐dodecen‐1‐yl acetate ( Z 8‐12:Ac), respectively. The atomization of 0.125, 0.25, 0.5 or 1 μL ethanol min −1 into the EAG air delivery tube at an airflow rate of 2 L min −1 , with resultant concentrations of 6.25, 12.5, 25 or 50 × 10 −5 μL ethanol mL air −1 , respectively, does not affect the EAG response of C. rosaceana or C. molesta after a 30‐min exposure period. The atomization of 0.125 μL min −1 of a solution of 8 mg Z 11‐14:Ac mL −1 ethanol into the EAG air delivery tube at an airflow rate of 2 L min −1 , with a resultant concentration of 0.5 ng pheromone mL −1 air, reduces the EAG response of C. rosaceana by approximately 70% after a 15‐min exposure period. An additional 15 min of exposure to pheromone does not result in increased sensory adaptation. Antennae recover 32% of the lost responsiveness when exposed to pheromone‐free air for 15 min. The atomization of 0.125 μL min −1 of a solution of 8 mg Z 8‐12:Ac mL −1 ethanol into the EAG air delivery tube at an airflow rate of 2 L min −1 , with a resultant concentration of 0.5 ng pheromone mL −1 air, reduces the EAG response of C. molesta antenna by approximately 80% after a 15‐ or 30‐min exposure period. The antennae of this species do not recover responsiveness when exposed to pheromone‐free air for 15 min.