Premium
High temperature causes arrest of cell cycle in G 2 phase in BmN cells derived from the silkworm, Bombyx mori
Author(s) -
KIUCHI TAKASHI,
AOKI FUGAKU,
NAGATA MASAO
Publication year - 2007
Publication title -
physiological entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 57
eISSN - 1365-3032
pISSN - 0307-6962
DOI - 10.1111/j.1365-3032.2006.00544.x
Subject(s) - bombyx mori , biology , cell cycle , microbiology and biotechnology , cell growth , cytoplasm , larva , dna synthesis , bombycidae , cell , cell culture , bombyx , dna , biophysics , biochemistry , botany , genetics , gene
The influence of temperature on the insect cell line, BmN, derived from the silkworm, Bombyx mori is investigated. These cells proliferate at an accelerated pace as the temperature increases from 22 to 30 °C, but the growth rate slows at 34 °C, and proliferation stops at 38 °C. At high temperatures, abnormal cellular morphology is observed. Cells treated at 38 °C have cytoplasmic bilateral protrusions and they gradually aggregate and float in the medium. BmN cells without proliferation at 38 °C are viable but have reduced DNA synthesis. At high temperatures, the cell cycle of BmN cells halts at the G 2 phase. After heat treatment of the larvae, an accumulation of larval haemocytes with high DNA content is found, which suggests that the cell cycle arrest at G 2 also occurs in the silkworm at high temperatures.