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Thermal conditioning of fifth‐instar Cydia pomonella (Lepidoptera: Tortricidae) affects HSP70 accumulation and insect mortality
Author(s) -
YIN X.,
WANG S.,
TANG J.,
HANSEN J. D.,
LURIE S.
Publication year - 2006
Publication title -
physiological entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 57
eISSN - 1365-3032
pISSN - 0307-6962
DOI - 10.1111/j.1365-3032.2006.00512.x
Subject(s) - tortricidae , codling moth , lepidoptera genitalia , biology , instar , hsp70 , larva , conditioning , horticulture , heat shock protein , polyacrylamide gel electrophoresis , botany , biochemistry , enzyme , statistics , mathematics , gene
Levels of HSP70 protein of fifth‐instar codling moth [ Cydia pomonella (L.) (Lepidoptera: Tortricidae)] are determined after conditioning at 35 °C for different times and also after recovery at 22 °C. Protein samples from larvae conditioned for different times are separated by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis electrophoresis. Sub‐lethal thermal conditioning at 35 °C for 40 min, 2, 6 and 18 h induces new protein bands in the extracts from treated codling moth larvae. Immunodetection with an antibody to a heat‐inducible HSP70 indicates a stronger reaction after 35 °C for 2, 6 and 18 h than after 35 °C for 40 min or control and, during the recovery period at 22 °C, the level of heat shock protein decreases. Conditioning of fifth‐instar codling moths at 35 °C also induces thermotolerance in the insects and necessitates longer times at a lethal temperature to ensure mortality. Thermotolerance is correlated with the accumulation of heat inducible HSP70 protein.