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Body extracts from the forest ant and honeybee: chromatographic comparison of factors affecting the ant Malpighian tubule
Author(s) -
Laenen Bart,
Wiehart Ursula,
De Decker Nadine,
Steels Paul,
Van Kerkhove Emmy
Publication year - 2003
Publication title -
physiological entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 57
eISSN - 1365-3032
pISSN - 0307-6962
DOI - 10.1111/j.1365-3032.2003.00349.x
Subject(s) - malpighian tubule system , biology , ant , antidiuretic , hymenoptera , secretion , aculeata , diuretic , chromatography , medicine , endocrinology , botany , hormone , chemistry , midgut , ecology , larva
. Crude trifluoroacetic acid extracts were prepared from different body parts of the forest ant, Formica polyctena Foerster, Hymenoptera, Formicidae, and the honeybee, Apis mellifera carnica Pollmann, Hymenoptera, Apidae. Extracts were prepurified by means of solid phase extraction over reversed‐phase cartridges. The effects of the resulting fractions on fluid secretion rates were tested on single preparations of ant Malpighian tubules. Diuretic and antidiuretic factors were shown to be present. High‐performance liquid chromatography allowed for the identification of a diuretic factor from the bee‐head extract which had a similar elution profile to a previously purified diuretic peptide from ant‐head extracts. An antidiuretic factor was also identified from the bee abdominal extract. Similar to an antidiuretic peptide, which was purified from ant abdominal extracts, this antidiuretic factor inhibited fluid secretion and depolarized the transepithelial potential of ant Malpighian tubules.