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Immunological evidence for FGLamide‐ and W 2 W 9 ‐allatostatins in the ovary of Gryllus bimaculatus (Ensifera, Gryllidae)
Author(s) -
Witek Günther,
Hoffmann Klaus H.
Publication year - 2001
Publication title -
physiological entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 57
eISSN - 1365-3032
pISSN - 0307-6962
DOI - 10.1111/j.1365-3032.2001.00217.x
Subject(s) - gryllus bimaculatus , biology , juvenile hormone , antiserum , polyclonal antibodies , ovary , orthoptera , microbiology and biotechnology , endocrinology , medicine , antibody , hormone , zoology , immunology , cricket
Summary Using polyclonal antisera to Gryllus bimaculatus allatostatin A1 (FGLamide) and B1 (W 2 W 9 ‐amide), two sensitive and specific competitive enzyme‐linked immunosorbent assays (ELISAs) were developed, which allowed detection of A‐ and B‐allatostatin‐like immunoreactivity in extracts of ovaries and partially purified chromatography (HPLC) fractions. Immuno‐positive HPLC fractions inhibited biosynthesis of Juvenile Hormone in vitro in the rapid partition assay. Both antisera were also used to immunolocalize A‐ and B‐allatostatin epitopes in the ovary of larval and adult crickets. A‐ and B‐allatostatin immunoreactivity appeared in the cortical cytoplasm of the oocytes at the anterior cell poles. Controls treated with the pre‐immune sera or with antisera preadsorbed onto the respective peptides coupled to a solid phase support did not stain. There was no staining in any neural structures within the ovary.