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Variability in Juvenile Hormone production by locust corpora allata kept in vitro for long periods
Author(s) -
GADOT M.,
PENER M. P.,
SCHAL C.
Publication year - 1993
Publication title -
physiological entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 57
eISSN - 1365-3032
pISSN - 0307-6962
DOI - 10.1111/j.1365-3032.1993.tb00597.x
Subject(s) - corpus allatum , juvenile hormone , biology , medicine , endocrinology , locust , stimulation , incubation , vitellogenesis , methoprene , schistocerca , hormone , biochemistry , oocyte , botany , embryo , microbiology and biotechnology
. Juvenile Hormone III (JH‐III) production by corpora allata (CA) of sexually mature female locusts (Locusta migratoria migratorioides (R. & F.)) was maintained in vitro for up to 30 days in an agar‐solidified medium. Hormone production was measured periodically with a short‐term radiochemical assay. Low‐activity CA increased their activity significantly after 24–48 h incubation in the long‐term medium, but high‐activity glands did not. Variations in activity were considerable among glands tested on the same day and among measurements from the same gland on different days. Farnesoic acid‐stimulated rates of JH‐III production were always higher than the basal rates, suggesting that the CA were not maximally activated. However, freshly excised low‐activity CA, whose hormone production increased in the long‐term conditions, showed similar farnesoic acid‐stimulated rates of JH‐III production to those of freshly excised high‐activity glands, suggesting that at the time of excision of the corpora allata rate‐limiting step(s) preceding farnesoic acid biosynthesis were inhibited or refractory to stimulation in vivo.