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Monitoring the effects of juvenile hormones and 20‐hydroxyecdysone on yolk polypeptide production of Drosophila melanogaster with enzyme immunossay
Author(s) -
WU SHUENNJUE,
ZHANG JIANZHONG,
MA MICHAEL
Publication year - 1987
Publication title -
physiological entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 57
eISSN - 1365-3032
pISSN - 0307-6962
DOI - 10.1111/j.1365-3032.1987.tb00761.x
Subject(s) - vitellogenesis , juvenile hormone , yolk , biology , hormone , medicine , methoprene , endocrinology , hemolymph , incubation , drosophila melanogaster , 20 hydroxyecdysone , corpus allatum , biochemistry , oocyte , microbiology and biotechnology , gene , embryo , ecology
. A double antibody sandwich enzyme‐linked immunosorbent assay (ELISA) was developed for detecting and quantifying small amounts of yolk polypeptides (YP) in studies on the hormonal control of vitellogenesis in Drosophila melanogaster Meigen. Monoclonal antibodies were incorporated as primary antibodies in the ELISA procedure to ensure selectivity in YP detection. The fact that YP concentration increases immediately after adult eclosion presents some difficulties in designing hormonal regulation experiments. Female adults decapitated immediately after eclosion remain alive for several days and virtually no YP is detected in the haemolymph 24 h after decapitation. The surgical procedure does not interfere with the competence of the fat bodies to respond to exogenous source of hormones. The effect of juvenile hormone (JH) on vitellogenesis can be studied by topical application of test material to these decapitated adults. A juvenile hormone analogue. Methoprene applied at 0.2 μg/fly or greater, restores YP production. The relative potencies of JH I 2 II 3 III and ZR 515 are compared at the same dose of 0.25 μg/fly. Their ranking in terms of re‐initiating vitellogenesis is ZR‐515 < JH II