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Unique roles of Schistosoma japonicum protein Sj16 to induce IFN‐γ and IL‐10 producing CD4 + CD25 + regulatory T cells in vitro and in vivo
Author(s) -
SUN X. J.,
LI R.,
SUN X.,
ZHOU Y.,
WANG Y.,
LIU X. J.,
LU Q.,
ZHOU C. L.,
WU Z. D.
Publication year - 2012
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2012.01377.x
Subject(s) - il 2 receptor , biology , foxp3 , immune system , immunology , schistosoma japonicum , population , interleukin 21 , microbiology and biotechnology , t cell , schistosomiasis , medicine , helminths , environmental health
Summary Various proteins are expressed during different stages of schistosome development that are essential for cercarial penetration of vertebrate skin and evasion of host immune response. CD4 + CD25 + regulatory T cells are important in modulating immune responses towards helminth infections. Schistosoma japonicum protein Sj16 present in the secretions of schistosomula has been shown to have anti‐inflammatory effects; however, it is uncertain whether Sj16 can induce CD4 + CD25 + regulatory T cells to participate in the regulation of early infection. In this study, we demonstrate a relationship between recombinant Sj16 (rSj16) and the induction of CD4 + CD25 + Foxp3 + regulatory T cells. An increase in CD4 + CD25 + T cells was observed both in splenic cells from mice injected with rSj16 and the cells pretreated with rSj16, respectively. The induced CD4 + CD25 + T cells suppressed CD4 + CD25 − T‐cell proliferation; furthermore, IFN‐γ and IL‐10 released from rSj16‐stimulated cells contribute to this suppression. Additionally, rSj16‐treated bone marrow dendritic cells (BMDCs) demonstrate an immature phenotype and play a role in the conversion of CD4 + CD25 − T cells into suppressive CD4 + CD25 + regulatory T cells. Our study identified a new CD4 + CD25 + T‐cell population that induced by rSj16 and suggests that an IFN‐γ‐biased microenvironment during early infection of schistosome may favour the establishment of infection.