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Trichinella spiralis‐ secreted products modulate DC functionality and expand regulatory T cells in vitro
Author(s) -
ARANZAMENDI C.,
FRANSEN F.,
LANGELAAR M.,
FRANSSEN F.,
Van Der LEY P.,
Van PUTTEN J.P.M.,
RUTTEN V.,
PINELLI E.
Publication year - 2012
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2012.01353.x
Subject(s) - trichinella spiralis , biology , foxp3 , il 2 receptor , trichinella , immune system , microbiology and biotechnology , immunology , signal transduction , in vitro , toll like receptor , regulatory t cell , secretion , t cell , tlr4 , t cell receptor , antigen , helminths , innate immune system , endocrinology , biochemistry
Summary Helminths and their products can suppress the host immune response which may benefit parasite survival. Trichinella spiralis can establish chronic infections in a wide range of mammalian hosts including humans and mice. Here, we aim at studying the effect of T. spiralis muscle larvae excretory/secretory products (TspES) on the functionality of DC and T cell activation. We found that TspES suppress in vitro DC maturation induced by both S‐ and R‐form lipopolysaccharide(LPS) from enterobacteria. Using different toll‐like receptor (TLR) agonists, we show that the suppressive effect of TspES on DC maturation is restricted to TLR4. These helminth products also interfere with the expression of several genes related to the TLR‐mediated signal transduction pathways. To investigate the effect of TspES on T cell activation, we used splenocytes derived from OVA‐TCR transgenic D011.10 that were incubated with OVA and TspES‐pulsed DC. Results indicate that the presence of TspES resulted in the expansion of CD4 + CD25 + Foxp3+ T cells. These regulatory T (Treg) cells were shown to have suppressive activity and to produce TGF‐β. Together these results suggest that T. spiralis secretion products can suppress DC maturation and induce the expansion of functional Treg cells in vitro .