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CTL responses to Leishmania mexicana gp63‐cDNA vaccine in a murine model
Author(s) -
ALI S. A.,
REZVAN H.,
MCARDLE S. E.,
KHODADADI A.,
ASTEAL F. A.,
REES R. C.
Publication year - 2009
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2009.01111.x
Subject(s) - ctl* , leishmania mexicana , gene gun , cytotoxic t cell , biology , leishmania major , immunization , cytotoxicity , immunology , immune system , antigen , leishmania , complementary dna , splenocyte , immunity , virology , microbiology and biotechnology , dna vaccination , gene , cd8 , in vitro , parasite hosting , genetics , computer science , world wide web
Summary Immunity to Leishmania is believed to be strongly dependent upon the activation of Th1 immune responses, although the exact role of cytotoxic T lymphocytes (CTLs) has not yet been determined. The aims of this study were to establish a suitable cytotoxicity assay to measure CTL activity and to compare immunity induced by Leishmania mexicana gp63 cDNA via i.m. injection and gene gun immunization in the BALB/c mouse model. The CTL activity was evaluated by short‐term 51 Cr‐release cytotoxicity assays against CT26 tumour cells transfected with L. mexicana gp63 cDNA and dendritic cells (DCs) loaded with soluble Leishmania antigen (SLA) as targets. The results clearly demonstrated that higher protection to L. mexicana infection was induced by gene gun DNA‐immunization vs. i.m. injection. Cytotoxic T lymphocyte activity of splenocytes was observed in mice immunized either with L. mexicana gp63 cDNA or SLA and long‐lived CTL activity was observed in immunized and/or re‐challenged mice but not naïve mice infected with the parasite.