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In vitro antigen presenting cell‐derived IL‐10 and IL‐6 correlate with Trichuris muris isolate‐specific survival
Author(s) -
D’ELIA R.,
ELSE K. J.
Publication year - 2009
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2008.01088.x
Subject(s) - biology , cd11c , antigen , immunology , immune system , in vitro , cytokine , microbiology and biotechnology , interleukin 10 , phenotype , gene , biochemistry
SUMMARY Trichuris muris , the mouse whipworm, is used as a laboratory model of the human parasite T. trichiura . Three laboratory isolates of T. muris exist — the E, J and S isolates. Previous data have shown that the S isolate survives to chronicity in C57BL/6 mice unlike the E and J isolates, which are expelled. The ability of the S isolate to persist is thought to be due to it secreting unique excretory/secretory antigens, which interact with APCs such that protective T cell responses do not develop. To determine whether APCs respond differently to E/S antigens from the three isolates we cultured isolate‐specific E/S with bone marrow‐derived macrophages (BMMΦ) and dendritic cells (BMDCs) in vitro . Markers of co‐stimulation and levels of MHC‐II were analysed by FACS and cytokine levels in supernatants quantified. E/S antigens from the S isolate consistently stimulated significantly higher levels of IL‐10 and IL‐6 from both macrophages (F4/80 + CD11b + CD11c − ) and dendritic cells (CD11c + CD11b + F4/80 − ) compared to J and E isolate E/S. If these in vitro differences in APC‐derived cytokines, particularly IL‐10, are biologically significant in vivo , they may contribute to the S isolate survival, by creating a regulatory cytokine environment in which protective immune responses are less effective.