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Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production
Author(s) -
PANADERO R.,
DACAL V.,
LÓPEZ C.,
VÁZQUEZ L.,
CIENFUEGOS S.,
DÍAZ P.,
MORRONDO P.,
DÍEZBAÑOS P.
Publication year - 2009
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2008.01072.x
Subject(s) - biology , concanavalin a , antigen , peripheral blood mononuclear cell , cytokine , immunology , immune system , in vitro , stimulation , lymphocyte , interleukin 2 , endocrinology , biochemistry
SUMMARY This study examines the immunomodulatory effect of a crude larval extract (CLE), obtained from first stage larvae (L1) of H. lineatum , and the purified fractions hypodermin A (HyA), HyB and HyC. Proliferative responses of peripheral blood mononuclear cells (PBMC) from uninfested and previously infested cattle and the production of the cytokines IL‐10, IL‐4 and IFN‐γ, in response to concanavalin A (Con A), were determined. The stimulation index of peripheral blood mononuclear cells from uninfested cattle was significantly lower than that from infested animals with the different antigens assayed. The HyA was the antigen that most inhibited the proliferative response, followed by the HyB, the HyC and the CLE. This hypodermin provoked an increase of IFN‐γ and a suppression of IL‐10 production that would support a Th1‐like cytokine response. The HyB reduced the production of IL‐10 stimulated by the Con A in cultures from infested animals. The HyC did not modulate the production of cytokines. Finally, the CLE induced a marked suppression in the production of the different cytokines in cultures from naïve and previously sensitized animals. Our results indicate that Hypoderma larval secretions are comprised of different components (hypodermins) that individually induce distinct but partially overlapping modulatory responses.