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Mast cells are activated by Leishmania mexicana LPG and regulate the disease outcome depending on the genetic background of the host
Author(s) -
VILLASEÑORCARDOSO M. I.,
SALAIZA N.,
DELGADO J.,
GUTIÉRREZKOBEH L.,
PÉREZTORRES A.,
BECKER I.
Publication year - 2008
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2008.01042.x
Subject(s) - biology , degranulation , immunology , immune system , inflammation , mast cell , tlr2 , leishmania mexicana , cutaneous leishmaniasis , bone marrow , cytokine , leishmaniasis , leishmania , innate immune system , parasite hosting , receptor , biochemistry , world wide web , computer science
SUMMARY The regulatory effect of mast cells on the pathogenesis of leishmaniasis is unclear. We report a comparative analysis of TLR2 membrane expression, TNF‐α, IL‐10 and MIP‐1α production, and granule release of bone marrow‐derived mast cells (BMMCs) from susceptible BALB/c and resistant C57BL/6 mice, stimulated in vitro with Leishmania mexicana lipophosphoglycan (LPG). We studied the kinetics of mast cell degranulation and parasite numbers in lesions of both mouse strains infected with L. mexicana . We found that BMMCs of C57BL/6 mice expressed more TLR2 and produced higher levels of both cytokines and MIP‐1α, whereas BALB/c BMMCs significantly augmented their granule release. Lesions of BALB/c mice showed higher levels of degranulated mast cells at 3 h of infection, whereas after 3 days of infection, the number of degranulated mast cells in C57BL/6 was higher than in BALB/c lesions. Throughout infection, BALB/c mice harboured more parasites. The regulatory effect of mast cells seems to depend on the genetic background of the host: mast cells of BALB/c mice facilitate disease progression due to an augmented inflammatory response early in the infection, whereas mast cells of C57BL/6 mice produce cytokines that regulate inflammation and maintain an elevated number of immune cells in the lesions, promoting disease control.