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Processing of the circumsporozoite protein in infected hepatocytes is not dependent on aspartic proteases
Author(s) -
BONGFEN S. E.,
BALAM S.,
TORGLER R.,
ROMERO J. F.,
CORRADIN G.
Publication year - 2008
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2008.01032.x
Subject(s) - circumsporozoite protein , biology , plasmodium berghei , proteases , immune system , antigen , plasmodium (life cycle) , virology , in vitro , antigen processing , immunology , aspartic acid , cd8 , microbiology and biotechnology , epitope , malaria , biochemistry , parasite hosting , enzyme , amino acid , mhc class i , world wide web , computer science
SUMMARY CD8 + T cells play a major role in the protective immune response against the liver stage of malaria. It was previously shown that the circumsporozoite protein (CSP) is processed and presented to specific T cells by both traversed and infected hepatocytes, but their respective antigen processing requirements were not completely defined. In the present study, we show that in vitro processing of the Plasmodium berghei CSP by infected mouse primary hepatocytes is exclusively dependent on proteasomes, while aspartic proteases are also needed in the case of traversed hepatocytes.