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Protective effect of a multiantigenic DNA vaccine against Toxoplasma gondii with co‐delivery of IL‐12 in mice
Author(s) -
CUI Y.L.,
HE S.Y.,
XUE M.F.,
ZHANG J.,
WANG H.X.,
YAO Y.
Publication year - 2008
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.1365-3024.2008.01025.x
Subject(s) - dna vaccination , toxoplasma gondii , biology , adjuvant , immunology , virology , antigen , toxoplasmosis , plasmid , balb/c , antibody , immune system , immunization , dna , genetics
SUMMARY In this study, we constructed a multiantigenic DNA vaccine, pSAG1‐ROP2‐SAG2 and examined its effect with co‐delivery of a plasmid encoding IL‐12 (pIL‐12) as an adjuvant in BALB/c mice against Toxoplasma gondii . After a lethal challenge of T. gondii RH strain, survival of the mice immunized with this pSAG1‐ROP2‐SAG2 vaccine was significantly prolonged in comparison to the control groups. Furthermore, the protection was significantly augmented by pIL‐12 co‐delivery. As demonstrated by lymphocyte proliferation assay, cytokine and antibody level determinations, the humoral and Th1‐type cellular responses elicited by this multiantigenic DNA vaccine were significantly stronger than those elicited by double‐antigenic, or single‐antigenic DNA vaccines. Our data suggest that multiantigenic DNA vaccine with pIL‐12 co‐delivery is a very effective approach in the protection against T. gondii.